| 摘要 |
Methods for the electrochemical detection of target analytes using a porous substrate and related systems are provided. In some embodiments, an electrochemical assay comprises determining the presence, absence, and/or concentration of one or more target analyte based on the electrical potential of an electroactive moiety (EAM) comprising a self-immolative moiety (SIM). In some embodiments, at least a portion of the electrochemical assay may occur within, on, and/or near a porous substrate. In some such embodiments, one or more component of the electrochemical assay (e.g., capture ligand, enzyme) may be immobilized within and/or on the porous substrate. In some embodiments, the immobilization of one or more assay components within and/or on the porous substrate may allow for the detection of multiple target analytes in a single sample as well as enhance assay performance. |
