ANTIBIOTIC-RESISTANT BACTERIA AND THEIR USES

摘要

Provided are novel bacteria that have increased antibiotic resistance. In particular, the bacteria have increased resistance to quinolone antibiotics. The bacteria of the invention include mutations selected from the following group: a mutation of GyrA; a mutation of OmpR; a mutation of YfeN; a mutation of ParC; a mutation of SoxR; a mutation of GrlA; a mutation of GrlB; an intergenic mutation upstream of NorA; a mutation of MgrA; a mutation of PBP2; a mutation of LyrA; a mutation of hypothetical probable lipoprotein (homologous to ORF SAOUHSC 01584 of S. aureus); a mutation of hypothetical probable membrane protein (homologous to ORF SAOUHSC 00508 of S. aureus); a mutation of MoaA; a mutation of NanM; a mutation of hypothetical glutathione ABC transporter ATP-binding protein (ORF DR76 1147 of E. coli ATCC 25922); a mutation of hypothetical transcription regulator of the LysR-family (ORF DR76 208 of E. coli ATCC 25922); a mutation of hypothetical L-serine dehydratase (ORF DR76 3029 of E. coli ATCC 25922); a mutation of GntU; and a mutation of PstA. Also provided are methods of generating bacteria with increased antibiotic resistance, as well as methods of identifying a compound with antibiotic activity. The invention further relates to methods utilising such bacteria, including methods for the design, discovery or development of antibiotic compounds, including new antibiotic compounds.