METHOD FOR DETECTING AND QUANTIFYING A TARGET ANALYTE IN A SAMPLE

摘要

The invention relates to a method for identifying and quantifying by mass spectrometry at least one target analyte in a sample, comprising the following steps:
a) depositing the sample to be analyzed on a support;b) analyzing the sample by mass spectrometry, so as to obtain the mass spectrum of the target analyte in said sample;c) weighting a signal associated with the mass spectrum of the target analyte in said sample by a extinction coefficient (TEC) specific to the target analyte and to the sample; andd) using the weighted signal of the target analyte to determine the quantity of target analyte in the sample.

主权项

1. A method for detecting and quantifying by mass spectrometry imaging at least one target analyte in a sample, comprising the following steps:
a) depositing the sample containing at least one target analyte to be analyzed on a support for mass spectrometry imaging and depositing a known concentration of isotopically labeled target analyte, as an internal standard, on said sample; b) analyzing the sample by a mass spectrometry imaging technique so as to obtain a mass spectrum signal of said at least one target analyte and a mass spectrum signal of the isotopically labeled target analyte in said sample; c) weighting the signal associated with the mass spectrum of said at least one target analyte in said sample by an extinction coefficient specific to each of said at least one target analyte and to the sample, wherein said extinction coefficient corresponds to a ratio of the signal of the at least one target analyte to the signal of the isotopically labeled target analyte, or the inverse; and d) using each weighted signal of each of said at least one target analyte to determine the quantity and distribution of each of said at least one target analyte in the sample.