SINGLE CELL CAPTURE WITH CAPTURE CHIPS

摘要

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and/or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.

主权项

1. A system comprising:
a) a capture chip comprising a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell mixture; and b) a multi-well through-hole chip, wherein said multi-well through-hole chip comprises a plurality of holes, and when combined with a backing or said capture chip, forms a multi-well chip which comprises a plurality of wells; and wherein said plurality of cell-sized dimples or wells matches one-for-one, and aligns with, said plurality of holes in said multi-well through-hole chip.