A fluorometer for measuring fluorescence of a sample includes an excitation source for emitting electromagnetic radiation along a first beam path to induce fluorescence in the sample. An excitation filter transmits electromagnetic radiation from the excitation source toward the sample. An excitation filter holder supports the excitation filter and defines an aperture for passage of electromagnetic radiation from the excitation source. The aperture is positioned asymmetrically relative to the first beam path such that the aperture allows an asymmetrical portion of the electromagnetic radiation in the first beam path to pass toward the sample and the excitation filter holder blocks passage of a corresponding asymmetrical portion of the electromagnetic radiation in the first beam path. The blocked passage of the corresponding asymmetrical portion of the electromagnetic radiation in the first beam path reduces the amount of electromagnetic radiation oriented directly from the emitter module to the detector module.
