Depletion of mouse cells for generic isolation of human cells upon xenotransplantation

摘要

Object of the invention is a process for depleting host cells from a xenograft of human cells on a murine host characterized in
a) fragmenting the xenograft into a sample comprising a single cell suspension
b) subjecting the sample to antibodies specific for a murine CD9 epitope coupled to a detection means
c) depleting the cell suspension from cells bound by the CD9-antibodies using the detection means
d) collecting the cells not bound by the CD9-antibodies as target cells.