| 摘要 |
The present invention establishes a method for the covalent anchoring of deoxyribonucleic acid extracted from eukaryotic cells, specifically products of polymerase chain reaction, over gold substrates. This method has the advantage of being quick, easy, marking free, affordable and may be used for the potential development of biosensors of deoxyribonucleic acid. The covalent anchoring method for deoxyribonucleic acid provided in the invention is formed by several steps: the first step refers to the formation of a self-assembly monolayer of 6-mercapto-1-hexanol over the gold substrate, the second step being the activation of said monolayer with the compounds N-3-Dimethylaminopropyl-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, the third step including the location of the polymerase chain reaction products over the gold substrate, previously purified and denaturalized. The final step consists in the exposure of the gold substrate with the polymerase chain reaction product s to the UV light for 3 min; this last step allows the covalent link between the polymerase chain reaction products and the monolayer of 6-mercapto-1-hexanol to be reinforced. One of the main applications of the inventive anchoring method is the determination of mutations or polymorphisms performed by directly using the deoxyribonucleic acid of patients, which has a greater number of applications above all in the diagnosis of a variety of diseases. |
