Transducible delivery of nucleic acids using modified dsRNA binding domains

摘要

The disclosure provides fusion polypeptides and constructs useful in delivering anionically charged nucleic acid molecules including diagnostics and therapeutics to a cell or subject. The fusion constructs include a protein transduction domain and a nucleic acid binding domain, or a protein transduction domain and a nucleic acid that is coated with one or more nucleic acid binding domains sufficient to neutralize an anionic charge on the nucleic acid. Also provided are methods of treating disease and disorders such as cell proliferative disorders.

主权项

1. A composition comprising a nucleic acid binding polypeptide containing the sequence selected from the group consisting of:
(a) SEQ ID NO:2, wherein at least 2 histidines are present in the sequence at a position selected from residues selected from the group consisting of 16, 18, 19, 20, 37, 38, 44, 46, 57 and 58; (b) SEQ ID NO:3; (c) SEQ ID NO:4; (d) SEQ ID NO:5; and (e) SEQ ID NO:6, wherein the polypeptide is in complex with an anionically charged nucleic acid to form a nucleic acid binding protein-nucleic acid complex having a net cationic charge.