| 主权项 |
1. A method for identifying the presence of Mycoplasma hyopneumoniae bacterium strain ts-19 in a sample, the method comprising:
assaying the sample comprising Mycoplasma hyopneumoniae bacteria for the presence or absence of a nucleic acid molecule with a mutation selected from the group consisting of: a GidA gene [tRNA uridine 5-carboxymethylaminomethyl modification enzyme] mutation, wherein an adenine at nucleotide 3075 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 7324 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 17143 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; an isoleucyl tRNA synthetase mutation, wherein a thymine at nucleotide 31773 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a GTPase ObgE mutation, wherein an adenine at nucleotide 48566 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 48881 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a DNA polymerase IV mutation, wherein an adenine at nucleotide 51166 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 59567 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 66347 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein an adenine at nucleotide 75037 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; an exonuclease ABC subunit C mutation, wherein a thymine at nucleotide 79461 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 86810 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 89715 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 94740 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein a thymine at nucleotide 94742 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein a thymine at nucleotide 104392 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 104393 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 117390 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein an adenine at nucleotide 118681 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 118716 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 120943 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present; a putative outer membrane protein P95 mutation, wherein an adenine at nucleotide 125147 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a triosephosphate isomerase mutation, wherein an adenine at nucleotide 131715 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 133772 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 147361 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein a thymine at nucleotide 153557 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein an adenine at nucleotide 155557 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 157051 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a uracil-DNA glycosylase mutation, wherein an adenine at nucleotide 163824 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 170370 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a putative chromate transport protein mutation, wherein a thymine at nucleotide 178555 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 183981 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 187424 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 187449 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a putative ABC transporter ATP binding and permease protein mutation, wherein an adenine at nucleotide 190701 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a 50S ribosomal protein L3 mutation, wherein a thymine at nucleotide 212677 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein an adenine at nucleotide 214047 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 214362 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein a thymine at nucleotide 226284 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 234616 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 235499 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence or a putative lipoprotein mutation, wherein an adenine at nucleotide 246806 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present; a putative lipoprotein (MHJ—0213) mutation, wherein an adenine at nucleotide 246808 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a ribonucleotide-disulphate reductase subunit alpha mutation, wherein a thymine at nucleotide 252401 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 271614 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 293668 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a thymidylate kinase mutation, wherein an adenine at nucleotide 300092 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a thymidylate kinase mutation, wherein an adenine at nucleotide 300098 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a DNA polymerase III subunit delta mutation, wherein an adenine at nucleotide 300772 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a DNA ligase mutation, wherein a thymine at nucleotide 305732 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a CDP-diacylglycerol-3-phosphate-3-phosphatidyl transferase mutation, wherein an adenine at nucleotide 328394 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 410563 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein a thymine at nucleotide 412710 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 413717 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 426313 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 431650 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein a thymine at nucleotide 431669 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a putative ABC transport system permease protein p69-like mutation, wherein a thymine at nucleotide 437234 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a putative lipoprotein (MHJ—0362) mutation, wherein an adenine at nucleotide 442618 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a putative ABC transporter permease protein mutation, wherein an adenine at nucleotide 465533 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 491786 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein a thymine at nucleotide 502594 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein a thymine at nucleotide 512112 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a transketolase mutation, wherein an adenine at nucleotide 519298 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 534108 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 539244 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein a thymine at nucleotide 558831 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 564250 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein an adenine at nucleotide 569870 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 586142 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein a thymine at nucleotide 591871 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 608132 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 613911 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in a hypothetical protein, wherein a thymine at nucleotide 615804 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a putative P216 surface protein mutation, wherein a TTG codon is inserted at nucleotides 625870-625872 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 inserting a glutamine into a protein encoded by the nucleic acid molecule; a putative p216 surface protein mutation, wherein a thymine at nucleotide 628031 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 634595 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; DNA gyrase subunit A mutation, wherein an adenine at nucleotide 683823 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein an adenine at nucleotide 699407 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 699923 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a thymine at nucleotide 737657 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is deleted causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 748454 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a ribonuclease H11 mutation, wherein an adenine at nucleotide 768571 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; an inorganic pyrophosphatase mutation, wherein an adenine at nucleotide 785221 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein an adenine at nucleotide 810058 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a mutation in an intergenic sequence, wherein an adenine at nucleotide 810061 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a putative ABC transporter ATP binding Pr1 mutation, wherein a thymine at nucleotide 817687 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a putative adhesion like protein P146 mutation, wherein a thymine at nucleotide 875082 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is not present causing a frameshift mutation in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence, wherein a thymine at nucleotide 883049 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO:5 is not present; a putative MgpA-like protein mutation, wherein a guanine at nucleotide 6060 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine; a mutation in a hypothetical protein, wherein a guanine at nucleotide 10909 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing an alanine to threonine substitution in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence between an amino acid permease and NADH oxidase, wherein an adenine at nucleotide 96181 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine; a mutation in a hypothetical protein, wherein an adenine at nucleotide 118293 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine causing an aspartate to glycine substitution in a protein encoded by the nucleic acid molecule; a putative outer membrane protein P95 mutation, wherein a guanine at nucleotide 123175 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a thymine causing a valine to phenylalanine substitution in a protein encoded by the nucleic acid molecule; a uracil-DNA glycolase mutation, wherein a guanine at nucleotide 163911 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing a serine to phenylalanine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a guanine at nucleotide 246307 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a thymine causing a glycine to cysteine substitution in a protein encoded by the nucleic acid molecule; a myo-inositol catabolism protein mutation, wherein a guanine at nucleotide 259905 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine; a DNA tropoisomerase I mutation, wherein an adenine at nucleotide 330948 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine; a DNA tropoisomerase I mutation, wherein an adenine at nucleotide 330998 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine causing a tyrosine to histidine substitution in a protein encoded by the nucleic acid molecule; a mutation in an intergenic sequence between a putative ISMHp1 transposase and a hypothetical protein, wherein a cytosine at nucleotide 379103 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine; a putative transposase truncated pseudo mutation, wherein a guanine at nucleotide 411019 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine; a mutation in a hypothetical protein, wherein a guanine at nucleotide 438867 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing a glycine to glutamine substitution in a protein encoded by the nucleic acid molecule; a putative ABC transporter permease protein mutation, wherein a cytosine at nucleotide 446756 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a adenine causing a leucine to isoleucine substitution in a protein encoded by the nucleic acid molecule; a nicotinate phosphoribosyltransferase mutation, wherein a guanine at nucleotide 495301 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a thymine causing a glycine to cysteine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a guanine at nucleotide 538653 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing an alanine to valine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a cytosine at nucleotide 550628 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing a glycine to valine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a guanine at nucleotide 554103 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing a proline to leucine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a guanine at nucleotide 555585 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a thymine causing a threonine to asparagine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a guanine at nucleotide 583967 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a thymosine causing a glycine to valine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a cytosine at nucleotide 599805 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing an aspartate to tyrosine substitution in a protein encoded by the nucleic acid molecule; a mutation in a hypothetical protein, wherein a cytosine at nucleotide 615445 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing a serine to isoleucine substitution in a protein encoded by the nucleic acid molecule; a putative PTS system N-acetylglucosmamine-specific II ABC component mutation, wherein a guanine at nucleotide 743961 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by an adenine causing an alanine to valine substitution in a protein encoded by the nucleic acid molecule; a leucyl-tRNA synthetase mutation, wherein a cytosine at nucleotide 848512 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine causing a methionine to isoleucine substitution in a protein encoded by the nucleic acid molecule; and a 16S ribosomal RNA MHJ—0709 mutation, wherein an adenine at nucleotide 881437 of a Mycoplasma hyopneumoniae J strain sequence provided as SEQ ID NO: 5 is substituted by a guanine; or assaying the sample comprising Mycoplasma hyopneumoniae bacteria for the presence or absence of a protein encoded by the nucleic acid molecule with the mutation, wherein the presence of the nucleic acid molecule with the mutation or the presence of the protein encoded by the nucleic acid molecule with the mutation indicates that the sample comprises the Mycoplasma hyopneumoniae bacterium strain ts-19. |
