Method and kit for detection of hemolytic uremic syndrome and necrotizing pneumonia in children with pneumococcal disease using serum fetuin-A levels as a biomarker

摘要

The invention provides a method and a kit for measuring the levels of serum fetuin-A in children to reflect the severity of invasive pneumococcal diseases caused by Streptococcus pneumoniae, including necrotizing pneumonia and hemolytic uremic syndrome. The mean fetuin-A levels in the HUS patients are significantly lower (207±80 mg/L, p<0.001) when compared to patients with lobar pneumonia (610±190 mg/L) and the healthy controls (630±250 mg/L). A serum fetuin-A level is a useful biomarker to classify the severity of pneumococcal infection in children. The level of biomarker fetuin-A can be monitored and evaluated by enzyme linked immunosorbent assay and Western blot hybridization.

主权项

1. A method of detecting an invasive pneumococcal infection with the associated complication of hemolytic uremic syndrome (HUS) or necrotizing pneumonia in a pediatric patient having the infection, the method comprising:
(a) collecting a serum sample from the pediatric patient having the invasive pneumococcal infection; (b) determining the level of sialoglycoprotein fetuin-A in the serum sample, and (c) comparing the serum fetuin-A level present in healthy pediatric persons with the fetuin-A level in the serum sample of the pediatric patient having the invasive pneumococcal infection with the associated complication of HUS or the necrotizing pneumonia, wherein the mean serum fetuin-A level in the healthy pediatric persons is 630±250 mg/L, wherein the mean serum fetuin-A level of 207±80 mg/L in the pediatric patient is indicative that the patient has the invasive pneumococcal infection with the associated HUS and wherein the mean serum fetuin-A level of 390±220 mg/L in the pediatric patient is indicative that the patient has the invasive pneumococcal infection with the associated necrotizing pneumonia.