Methods for detecting organisms and enzymatic reactions using raman spectroscopy

摘要

The present disclosure provides systems for the rapid and sensitive detection of organisms and molecules in samples. Reactants that produce Raman-active products are used in combination with Raman light scattering. The present disclosure can also be used to measure enzyme-kinetics.

主权项

1. A method for detecting the activity of at least one enzyme in a sample comprising:
a) preparing a mixture comprising the sample and:
i. optionally at least one aromatic compound;ii. at least one amine-containing compound; andiii. at least one electron-donating compound; b) incubating the mixture in the presence of a base to form at least one Raman-active product; and c) detecting the at least one Raman-active product with Raman spectroscopy, wherein the method does not comprise Surface Enhanced Resonance Raman Scattering (SERRS).