Chemically modified cellulose fibrous meshes for use as tissue engineering scaffolds

摘要

Cellulose and sulfated cellulose fibrous meshes exhibiting robust structural and mechanical integrity in water were fabricated using a combination of electrospinning, thermal-mechanical annealing and chemical modifications. The sulfated fibrous mesh exhibited higher retention capacity for human recombinant bone morphogenetic protein-2 than the cellulose mesh, and the retained proteins remained biologically active for at least 7 days. The sulfated fibrous mesh also more readily supported the attachment and osteogenic differentiation of rat bone marrow stromal cells in the absence of osteogenic growth factors. These properties combined make the sulfated cellulose fibrous mesh a promising bone tissue engineering scaffold.

主权项

1. A fibrous mesh of thermal-mechanically annealed cellulose that is subsequently oxidized and sulfated,wherein the thermal-mechanical annealing is performed under a pressure in the range from about 10 MPa to about 500 MPa and at a temperature from about 60° C. to about 240° C.; and wherein
the fibrous mesh have thicknesses from about 10 μm to about 500 μm and comprise fibers from about 100 nm to about 20 μm in fiber diameter; the fibrous mesh comprises fibers having a tensile elastic modulus from 2 MPa to 20 MPa and an ultimate tensile strength from 50 KPa to 1.0 MPa.