Flagellin fusion proteins and use thereof to induce immune responses against <i>Pseudomonas aeruginosa</i>

摘要

The invention provides compositions and fusion proteins comprising a flagellin adjuvant and a Pseudomonas aeruginosa antigen. The invention further provides pharmaceutical formulations and methods for inducing an immune response against P. aeruginosa (e.g., to prevent and/or treat P. aeruginosa infection).

主权项

1. A nucleic acid encoding a fusion protein comprising: (a) a Pseudomonas aeruginosa type A flagellin adjuvant or a Pseudomonas aeruginosa type B flagellin adjuvant; and (b) a Pseudomonas aeruginosa antigen, the antigen comprising a Pseudomonas aeruginosa OprF antigen and a Pseudomonas aeruginosa OprI antigen, wherein the Pseudomonas aeruginosa antigen is an N-terminal extension of the flagellin adjuvant, wherein the fusion protein is able to activate the TLR5 pathway, and wherein there is no significant difference in the half-maximal stimulation of TNF-α production between Pseudomonas aeruginosa type A flagellin, Pseudomonas aeruginosa type B flagellin, and the fusion protein.