fragmento de ácido nucleíco, construto de ácido nucleíco, vetor de expressão, célula hospedeira, método de geração de uma célula hospedeira para a expressão de um produto gênico de interesse, e, método de expresão de um produto de gene de interesse

摘要

The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.