診断の方法を行うためのニトロ化タンパク質又はペプチド配列の使用

摘要

Quantitative determination of protein tyrosine nitration levels is used to determine the severity and progressive nature of a disease associated with nitration stress. Independent claims are also included for: (1) antibodies specific for albumin with nitrated tyrosine at position 138 or 411; (2) determining the severity and progressive nature of a disease associated with nitration stress by quantitatively determining the nitration level of a protein P1 by comparing the concentration of nitrated P1 with the total concentration of nitrated and nonnitrated P1 and comparing this nitration level with the nitration level of P1 in a sample from an individual that is not disease-affected; (3) preparing a protein with the same nitrated tyrosine residues as a nitrated protein occurring in a disease associated with nitration stress by reacting a protein with tetranitromethane in a tetranitromethane:tyrosine molar ratio of up to 20:1 in an aqueous buffer with a pH of 7.5-8.5 and identifying the nitrated tyrosines, especially by mass spectrometry.