Conformational-switching fluorescent protein probe for detection of alpha synuclein oligomers

摘要

A conformation-switching fluorescent protein probe for detection of alpha synuclein oligomers using an alpha synuclein (αS) variant, PG65 (SEQ ID NO: 4), together with a conformation-sensitive fluorescent molecule to create a molecular probe for rapid, specific, and quantitative detection of αS oligomers.

主权项

1. A kit for detecting αS oligomers, the kit comprising a protein comprising the sequence:(SEQ ID NO: 4)MDVFMKGLSKAKEGWAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGWHGVATVAEKTKEQVTCCPGCCAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA.