Vaccine comprising protein NMB0964 from <i>Neisseria meningitidis </i>

摘要

The present invention relates to immunogenic compositions comprising neisserial blebs with upregulated levels of the NMB0964 antigens such that bacterial antibodies are generated against said antigen. Methods are also provided to upregulate expression through removal of the zinc repression mechanism of the cell or promoter or through removal of zinc from the culture medium.

主权项

1. An immunogenic composition comprising:
(a) a pharmaceutically acceptable excipient and (b) isolated outer membrane vesicles enriched in the zinc-regulated NMB0964 polypeptide of Neisseria meningitidis, wherein the isolated outer membrane vesicles are from a Neisseria meningitidis that is genetically modified through the disruption of functional expression of its Zur repressor protein, NMB1266, wherein the genetically modified Neisseria meningitidis produces an upregulated level of the NMB0964 polypeptide in the outer membrane vesicles, wherein said immunogenic composition when administered to a mammal in an immunologically effective amount, elicits antibodies specific to the NMB0964 polypeptide in the mammal.