| 摘要 |
PROBLEM TO BE SOLVED: To provide a novel method for preservation of microorganisms.SOLUTION: A method for preservation of microorganisms belonging to genus Mortierella comprises the steps of: (a) forming spores in a spore-forming medium at pH 4-7 containing a nutrient source comprising an inorganic salt and a saccharide; (b) suspending the spores obtained in the (a) in sterilized water, or sterilized water containing a surfactant and/or an inorganic salt to prepare a spore suspension, and adding a cryoprotectant at 5-15% to prepare a cryopreserving spore suspension, and (c) preserving the cryopreserving spore suspension obtained in the (b) at between -90°C and -30°C.SELECTED DRAWING: None |
