| 发明名称 |
SINGLE NUCLEOTIDE DETECTION METHOD |
| 摘要 |
A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets. |
| 申请公布号 |
US2016040224(A1) |
申请公布日期 |
2016.02.11 |
| 申请号 |
US201414782654 |
申请日期 |
2014.04.09 |
| 申请人 |
BASE4 INNOVATION LTD ;MEDICAL RESEARCH COUNCIL |
发明人 |
FRAYLING Cameron Alexander;BALMFORTH Barnaby;SOARES Bruno Flavio Nogueira de Sousa;ISAAC Thomas Henry;BREINER Boris;NATALE Alessandra;AMASIO Michele;DEAR Paul |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for determining the sequence of nucleotide bases in a polynucleotide analyte, the method comprising steps of:
(1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state; and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. |
| 地址 |
Cambridge, Cambridgeshire GB |
