主权项 |
1. A vital stain for observation under multiphoton laser microscopy, comprising one or more edible dye compounds that are USFDA (Food and Drug Administration)-approved or Japanese Ministry of Health, Labour and Welfare-approved food additives,
wherein the dye compound is selected from the group of fluorescent dye compounds including tar-based dyes, iridoid dyes, carotenoid-based dyes, flavonoid-based dyes, quinoid-based dyes and betalain-based dyes, the tar-based dyes being Red #3 (erythrosine), Red #104 (phloxine), Red #105, Red #106, Green #3 (Fast Green FCF) and Blue #2 (indigo carmine), the carotenoid-based dyes being annatto (annatto-N2R25, achiote fruit: bixin, norbixin), crocin G150 (Gardenia Yellow dye), crocin L (Gardenia Yellow dye), β-carotene and annatto-WA-20 (annatto dye achiote seed: norbixin), the flavonoid-based dyes being HI RED V80 (purple potato dye: cyanidin acyl glucoside and peonidin acyl glucoside), malvidin (blue sweet pea dye), tricetinidin (black tea dye), petunidine (red berry dye), curcumin, sulfuretin, myricetin (grape, onion dye) and quercetin (onion, citrus dye), the betalain-based dye being HI RED BL (red beet dye: betanin, isobetanin), and the vital stain stains luminal epithelial/adenocytic and/or connective tissue/capillary cancer cells. |