发明名称 |
METHOD FOR PRODUCING POLYHYDROXYALKANOATE COPOLYMER FROM SACCHARIDE RAW MATERIAL |
摘要 |
The purpose of the present invention is to provide a method for enhancing the production quantity of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)) having a high fraction of 3-hydroxyhexanoate (3HH) using a saccharide or glycerol as a starting material. The present invention provides: a method for producing a P(3HB-co-3HH) copolymer including performing transformation by homologous recombination of a crotonyl-CoA reductase gene in a chromosome of a recombinant strain of Cupriavidus necator endowed with the ability to produce P(3HB-co-3HH), or performing transformation by introducing an autonomous replication vector in which the crotonyl-CoA reductase gene is incorporated in the aforementioned strain, and propagating the transformant in a medium containing a saccharide or glycerol as a carbon source; and a method for enhancing the production quantity of the copolymer and/or the fraction of 3HHx in the copolymer. |
申请公布号 |
WO2016021604(A1) |
申请公布日期 |
2016.02.11 |
申请号 |
WO2015JP72107 |
申请日期 |
2015.08.04 |
申请人 |
TOKYO INSTITUTE OF TECHNOLOGY;KANEKA CORPORATION |
发明人 |
FUKUI, TOSHIAKI;ORITA, IZUMI |
分类号 |
C12P7/62;C12N9/02;C12N9/88;C12N15/09 |
主分类号 |
C12P7/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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