Method for Detecting and Monitoring Bone Loss

摘要

Methods for identifying subjects having bone loss by detecting bone microparticles in a sample of their bodily fluid are disclosed. Methods for monitoring bone loss and assessing efficacy of bone loss therapies by detecting bone microparticles in bodily fluid samples are also disclosed. Compounds for use as a negative control in the disclosed methods are provided as well as kits comprising such compounds.

主权项

1. A method for detecting bone loss in a subject, the method comprising:
a) measuring the amount of bone microparticles in a bodily fluid sample obtained from the subject, bone microparticles being fragments of bone originating from bone tissue, the measuring comprising: i) exposing the bodily fluid sample to at least one labeled binding probe that is specific to hydroxyapatite, hydroxyapatite being a biomarker of bone microparticles; and ii) measuring the amount of bone microparticles bound to the at least one labeled binding probe; b) comparing the measurement obtained in step (a) (ii) with a reference value; and c) detecting bone loss in the subject based on results of the comparison.