CONTINUOUS CULTURE FOR 1,3-PROPANEDIOL PRODUCTION USING HIGH GLYCERINE CONCENTRATION

摘要

The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content.;The invention also concerns an “adapted microorganism” which glycerol metabolism is directed to 1,3-propanediol production, and which is allowed to grow in the presence of a high concentration of industrial glycerine.;The invention also concerns a biosourced 1,3-propanediol obtained by the process thereof. Finally the invention concerns the use of the above described biosourced 1,3-propanediol as extender chain in thermoplastic polyurethane, as monomers in polytrimethylene terephtalate and as a component in cosmetics formulations.

主权项

1. A method for the production of 1,3-propanediol in a continuous fermentation process of glycerine, comprising the steps of:
a) culturing a strain of Clostridium on a synthetic culture medium comprising a high concentration of industrial glycerine, said industrial glycerine comprising more than 70% of glycerol and said glycerol being present at a concentration comprised between 90 and 120 g/L in said culture medium, and b) recovering the 1,3-propanediol from the culture medium, wherein: said culture medium does not contain any organic nitrogen source and said strain of Clostridium is genetically modified to have its glycerol metabolism directed to 1,3-propanediol production and is previously adapted by a continuous culture in the presence of a high concentration of industrial glycerine.