METHODS FOR MODULATING THE GLYCOSYLATION PROFILE OF RECOMBINANT PROTEINS USING DISSOLVED OXYGEN

摘要

The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of recombinant proteins expressed in host cells by supplementing the production media with dissolved oxygen.

主权项

1. A method of producing a composition comprising a recombinant protein with a modulated glycosylation profile, said method comprising:
culturing a host cell expressing said recombinant protein in cell culture media supplemented with dissolved oxygen, thereby producing said composition comprising said recombinant protein with a modulated glycosylation profile as compared to a control, wherein said control is a composition comprising said recombinant protein produced by culturing a host cell expressing said recombinant protein in cell culture media which is not supplemented with said dissolved oxygen.