LATERAL-FLOW ASSAY DEVICE WITH FILTRATION FLOW CONTROL

摘要

A lateral-flow assay device includes a substrate with a sample addition zone and a fluid flow path. A cover supports a filter having a portion contacting the substrate to create a contact area that at least partly overlaps the sample addition zone. Another portion extends from the contact portion to the supported periphery of the filter to define with the substrate a reservoir configured to retain the filtrate by capillary pressure between the substrate and the extending portion. The reservoir volume is based on an acute angle formed between the substrate and the extending portion of the filter and on a fluid meniscus of the filtrate. The filter and sample addition zone are configured to provide capillary pressure drawing the filtrate from the reservoir to the sample addition zone. Methods for controlling flow characteristics in a lateral-flow assay device are also described.

主权项

1. A lateral-flow assay device for a sample, the device comprising:
a) a substrate having a sample addition zone and a fluid flow path through which a filtrate flows under capillary action away from said sample addition zone; b) a cover arranged over the substrate and having an aperture defining a metering port configured to receive the sample; and c) a filter supported peripherally within the aperture and configured to permit at least a portion of the sample to pass therethrough as the filtrate, the supported filter having:
i) at least one contact portion in direct contact with the substrate to create a contact area that at least partly overlaps the sample addition zone; andii) another portion that extends from the at least one contact portion to the supported periphery of the filter to define with the substrate a reservoir configured to retain the filtrate by capillary pressure between the substrate and the extending portion of the filter, d) wherein the reservoir has a volume based on an acute angle formed between the substrate and the extending portion of the filter and based on a fluid meniscus of the filtrate, and the filter and sample addition zone are configured to provide a capillary pressure drawing the filtrate from the reservoir to the sample addition zone.