IMMUNOASSAYS OF S-ADENOSYLMETHIONINE AND METHYLATION INDEX IN PERSONALIZED MEDICINE AND HEALTH EVALUATION

摘要

The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

主权项

1. A method of assessing one or more health statuses of a subject, the method comprising: determining the methylation index status in a test sample from the subject; said methylation index being calculated by determining the levels of SAM and SAH using any immunoassays; comparing one or more of the determined methylation index states to one or more baseline reference methylation index states; wherein a difference, lack of a difference, or both in one or more of the determined methylation index states and one or more of the baseline reference methylation index states indicates one or more statuses of health of the subject.