| 发明名称 |
A HUMAN BLOOD-BRAIN BARRIER MODEL DERIVED FROM STEM CELLS |
| 摘要 |
The present disclosure relates to a method for obtaining human brain-like endothelial cells by contacting a population of cells isolated from stem cells with a differentiation medium to obtain endothelial cells and co-culturing said endothelial cells with pericytes, with cells of the neurovascular unit or with a pericytes conditioned medium, to obtain brain-like endothelial cells. The present disclosure also relates to the use of the brain-like endothelial cells as an in vitro model of human blood-brain barrier and a kit for measuring blood-brain barrier permeability of a substance, comprising in vitro human endothelial cells. |
| 申请公布号 |
US2016040125(A1) |
申请公布日期 |
2016.02.11 |
| 申请号 |
US201414780653 |
申请日期 |
2014.03.26 |
| 申请人 |
BIOCANT - CENTRO DE INOVAÇÃO EM BIOTECNOLOGIA ;UNIVERSITY OF ARTOIS |
发明人 |
DA SILVA FERREIRA Lino;SEVIN Emmanuel Bernard;CECCHELLI Roméo Paul;ADAY Sezin |
| 分类号 |
C12N5/079;G01N33/50 |
主分类号 |
C12N5/079 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for obtaining human brain-like endothelial cells comprising the following steps:
contacting a population of cells isolated from stem cells with a differentiation medium to obtain endothelial cells; co-culturing the said endothelial cells with pericytes or with cells of the neurovascular unit or with a pericytes conditioned medium, to obtain brain like endothelial cells. |
| 地址 |
Cantanhede PT |
