RECOMBINANT PRODUCTION OF HIV-1 ENVELOPE GLYCOPROTEINS
摘要
The present invention relates, in general, to HIV-1 and, in particular, to methods of producing HIV-1 envelope (Env) proteins, and subunits thereof, and to constructs suitable for use in such methods.
申请公布号
US2016039885(A1)
申请公布日期
2016.02.11
申请号
US201414781881
申请日期
2014.04.01
申请人
DUKE UNIVERSITY
发明人
Haynes Barton F.;Liao Hua-Xin;Yu Jae-sung
分类号
C07K14/005;C12N7/00
主分类号
C07K14/005
代理机构
代理人
主权项
1. A method of producing an HIV-1 Env protein that is resistant to cleavage when produced in CHO cells comprising substituting amino acid residues in the V3 loop of an HIV-1 Env protein that is cleaved when produced in CHO cells to yield a protein that has the V3 loop sequence TRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAH.
