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一种线粒体SNP荧光标记复合扩增试剂盒，其特征在于：包括有用于扩增如下61个SNP位点的引物：G10398A、Norm9bpDel、C10873T、A3010G、A709G、C7196A、C12705T、C3970T、G13104A、G10310A、C5178A、G13928C、G6446A、C8414T、C8794T、C8793T、C16126T、G16129A、A15043G、C16311T、A8701G、A8697G、C4883T、C10400T、(CA)n、G1719A、C14668T、C12811T、C,T9824A、C9698T、A9123G、C7028T、G11719A、G8584A、A11251G、G8020A、G5460A、G2706A、C11215T、T4216C、G12372A、C16362T、C1541T、C8684T、G9477A、A4491G、G1811A、G16316A、A16319G、G9545A、C11944T、C152T、G14569A、C8964T、G10397A、G3348A、G4833A、G7600A、G5417A、C5442T、C15784T；所述的引物的序列如下所示：A709G,SEQ ID NO.1‑3；A3010G,SEQ ID NO.4‑6；C5178A,SEQ ID NO.7‑9；Norm9bpDel,SEQ ID NO.10‑11；G10398A,SEQ ID NO.12‑14；C10873T,SEQ ID NO.15‑17；C12705T,SEQ ID NO.18‑20；G13928C,SEQ ID NO.21‑23；C16311T,SEQ ID NO.24‑26；A15043G,SEQ ID NO.27‑29；C3970T,SEQ ID NO.30‑32；C8414T,SEQ ID NO.33‑35；G6446A,SEQ ID NO.36‑38；C7196A,SEQ ID NO.39‑41；G10310A,SEQ ID NO.42‑44；G13104A,SEQ ID NO.45‑47；C8793T和C8794T,SEQ ID NO.48‑51；C16126T和G16129A,SEQ ID NO.52‑55；C4883T,SEQ ID NO.56‑58；C10400T,SEQ ID NO.59‑61；(CA)n,SEQ ID NO.62‑63；G11719A,SEQ ID NO.64‑66；G1719A和C1541T,SEQ ID NO.67‑71；G8584A和A8701G和A8697G,SEQ ID NO.72‑77；A9123G,SEQ ID NO.78‑80；C7028T,SEQ ID NO.81‑83；C14668T,SEQ ID NO.84‑86；G2706A,SEQ ID NO.87‑89；G12372A,SEQ ID NO.90‑92；T4216C,SEQ ID NO.93‑95；A11251G和C11215T,SEQ ID NO.96‑100；G5460A,SEQ ID NO.101‑103；C12811T,SEQ ID NO.104‑106；G8020A,SEQ ID NO.107‑109；C,T9824A,SEQ ID NO.110‑112；C9698T,SEQ ID NO.113‑115；C16362T,SEQ ID NO.116‑118；C152T,SEQ ID NO.119‑121；G1811A,SEQ ID NO.122‑124；A4491G,SEQ ID NO.125‑127；G4833A,SEQ ID NO.128‑130；G5417A和C5442T,SEQ ID NO.131‑135；G7600A,SEQ ID NO.136‑138；C8684T,SEQ ID NO.139‑141；C8964T,SEQ ID NO.142‑144；G9477A和G9545A,SEQ ID NO.145‑149；G10397A,SEQ ID NO.150‑152；G3348A,SEQ ID NO.153‑155；C11944T,SEQ ID NO.156‑158；G14569A,SEQ ID NO.159‑161；C15784T,SEQ ID NO.162‑164；G16316A和A16319G,SEQ ID NO.165‑168；引物在扩增体系中的终浓度是：<img file="FDA0000762784350000011.GIF" wi="2078" he="367" /><img file="FDA0000762784350000021.GIF" wi="2077" he="2313" /> |
