| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for producing a univesicular liposome which is high in drug inclusion rate, small in size, and sharp in particle size distribution, even when a two-step emulsification method is performed using a volatile hydrocarbon-based organic solvent as an oil phase (O). <P>SOLUTION: The method for producing the univesicular liposome of an average particle size of 50-200 nm includes: (1) a primary emulsification process in which a volatile oil phase liquid (O) obtained by dissolving or dispersing a lipid component (1) in a volatile hydrocarbon-based organic solvent (o) and an aqueous phase liquid (W1) obtained by dissolving an inclusion object drug (D) in an aqueous solvent (w1) are mixed and emulsified to prepare a W1/O emulsion; (2) a secondary emulsification process in which the W1/O emulsion obtained through the process (1) and an aqueous phase liquid (W2) including a non-crystalline lipid component added are mixed and emulsified to prepare a W1/O/W2 emulsion; (3) a solvent removal process in which the hydrocarbon-based organic solvent (o) is removed from the W1/O/W2 emulsion obtained through the process (2) to prepare a liposome dispersion; and (4) an aqueous phase substitution process in which the aqueous phase liquid (W2) is removed from the liposome dispersion obtained through the process (3) and an aqueous phase liquid (W3) is added to prepare a liposome preparation. <P>COPYRIGHT: (C)2013,JPO&INPIT |
