Directed surface functionalization on selected surface areas of topographical features with nanometer resolution

摘要

A method for making a single molecule receptor in a nanopore structure includes depositing a material by a physical vapor deposition (PVD) technique onto a selected interior surface of a nanochannel and functionalizing a surface of the material with a chemical compound having at least two functional groups. The material forms a patch having a diameter of about 3 to about 10,000 nanometers (nm). Also disclosed are embodiments of a nanopore structure including a single molecule receptor.

主权项

1. A nanopore structure comprising a single molecule receptor, the nanopore structure comprising:
a substrate having a first surface and an opposing second surface; a nanochannel extending from the first surface to the opposing second surface and defining an interior surface; a raised patch of a material disposed onto a selected area of the interior surface of the nanochannel, wherein the raised patch does not fully encompass the circumference of the nanochannel, the raised patch material having a surface area of about 3 to about 10,000 nm2 and extending from the selected area of the interior surface to the nanochannel's opening; and a chemical compound disposed onto the material to form a functionalized material that forms the single molecule receptor; wherein the single molecule receptor is a binding site with nanometer dimensions that binds to only a single molecule of an analyte in a complex sample.