Composition, method, and kit for calibrating a mass spectrometer

摘要

A composition, method, and kit for calibrating a mass spectrometer including a predetermined concentration of a calibrant having a mixture of amino acid polyethylene glycol compounds and a solvent for dissolving the calibrant. The calibrant can be used in either positive or negative ionization mode, and it can be used in calibrating an atmospheric pressure chemical ionization or an electrospray mass spectrometer. The calibrant can include a mixture to enable calibration across a broad range of masses.

主权项

1. A method for calibrating a mass spectrometer comprising:
a) obtaining a mass spectrum of a calibrant composition containing a plurality of known compounds comprising a mixture of discrete length polyethylene glycol compounds, each having an amino group on one end, a number of ethylene oxide units, and a carboxylic acid group on the other end, and a solvent for dissolving the calibrant; b) determining the differences between the expected mass peaks for the known compounds and the corresponding actual mass peaks obtained; c) adjusting the mass spectrometer based on the differences between the expected and actual mass peaks.