发明名称 |
PROCEDE DE DETECTION D'UNE ESPECE FLUORESCENTE REVERSIBLEMENT PHOTOCONVERTIBLE |
摘要 |
<p>Method for detection of at least one reversibly photo-convertible fluorescent species (P), comprising the following steps: a) illumination of a sample comprising said or at least one of said reversibly photo-convertible fluorescent species by a periodically modulated illuminating light (FEX); and b) detection of fluorescent radiation (FLU) emitted by the sample thus illuminated; characterised in that the method further comprises the following step: c) extraction of the amplitude (lF0Ut) of the intensity component of said fluorescent radiation exhibiting the same periodicity as said periodically modulated illuminating light and a phase quadrature with respect to the same; and in that the mean intensity of said illuminating light and the modulation frequency of the same are chosen in such a way as to maximise said amplitude of the intensity component of said fluorescent radiation.</p> |
申请公布号 |
FR3013452(B1) |
申请公布日期 |
2016.02.05 |
申请号 |
FR20130061476 |
申请日期 |
2013.11.21 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;INSTITUT CURIE;ECOLE NORMALE SUPERIEURE DE PARIS;UNIVERSITE PIERRE ET MARIE CURIE |
发明人 |
JULLIEN LUDOVIC;LE SAUX THOMAS;GAUTIER ARNAUD;CROQUETTE VINCENT;NATH SARANG;WANG PENGCHENG;QUERARD JEROME |
分类号 |
G01N21/64 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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