METHOD FOR PREPARING GLYCAN-HYDROLYZED ANTIBODY, AND HOMOGENEOUS GLYCOSYLATED ANTIBODY

摘要

The present invention is aimed to provide a method for preparing an acceptor that is N-glycan hydrolyzed antibody or a Fc fragment thereof used for producing antibody having a homogeneous N-glycan structure; a method for determining a combination of endoglycosidases for use in said preparation; and a method for measuring N-glycans linked to an antibody. The present invention is directed to a method for producing a N-glycan hydrolyzed antibody or Fc fragment thereof, comprising reacting the antibody or the Fc fragment thereof with several endoglycosidases; and a method for determining quantitative information of an objective N-glycan with a desired structure linked to an antibody or a Fc thereof, comprising a protease treatment step and a glycopeptide measurement step, etc.

主权项

1. A method for preparing a N-glycan hydrolyzed antibody or a Fc thereof, comprising reacting the antibody or the Fc thereof with several endoglycosidases.