METHODS FOR PROCESSING WHOLE BLOOD SAMPLES, AND COMPOSITIONS FOR USE IN PRACTICING THE SAME

摘要

Methods for processing whole blood samples are provided. Aspects of the methods include depleting leukocytes from a whole blood sample to produce a leukocyte depleted sample, and then lysing red blood cells (RBCs) in the resultant leukocyte depleted sample to produce a leukocyte/RBC depleted sample. Also provided are compositions and kits for practicing embodiments of the invention. The methods and compositions find use in a variety of different applications, including the detection of circulating tumor cells (CTCs).

主权项

1. A method for processing a whole blood sample, the method comprising:
depleting leukocytes from the whole blood sample via a leukocyte specific binding reagent mediated depletion protocol to provide a leukocyte depleted sample; and lysing red blood cells in the leukocyte depleted sample to provide a red blood cell (RBC) depleted sample.