GENERATION AND USE OF POLYCLONAL AND MONOCLONAL ANTIBODIES SPECIFIC FOR 3-PHOSPHOHISTIDINE
摘要
Isolated monoclonal antibodies and or antigen binding fragments thereof are disclosed herein that specifically bind polypeptides comprising a histidine phosphorylated at N3 (3-pHis). Nucleic acids encoding these antibodies, vectors including these nucleic acids, and host cells transformed with these vectors and nucleic acids are also disclosed. Methods are also disclosed for using these antibodies, such as for detection of polypeptides comprising a histidine phosphorylated at N3 (3-pHis). In some embodiments, the methods can be used to investigate signal transduction pathways.
申请公布号
WO2016018562(A1)
申请公布日期
2016.02.04
申请号
WO2015US38892
申请日期
2015.07.01
申请人
SALK INSTITUTE FOR BIOLOGICAL STUDIES;SANOFI
发明人
HUNTER, TONY;FUHS, STEPHEN, RUSH;MEISENHELDER, JILL;MAUGER, JACQUES;STANKOVA, MAGDA;AL-OBEIDI, FAHAD;BINNE, ROBERT, A.