IMPROVED METHOD OF MAKING EXTRACELLULAR MATRIX COMPOSITIONS

摘要

Provided herein are methods of producing extracellular matrix (ECM) that are superior to previously-described methods. Extracellular matrix (ECM) comprises protein that forms many structures in the body including tendons, ligaments, and sheets that support skin and internal organs. There remains a need in the art for ECM compositions that have improved cell attachment characteristics and methods of making such ECM compositions.

主权项

1. A method of producing an extracellular matrix (ECM) composition, comprising in order: decellularizing a tissue to produced decellularized ECM; freezing the ECM; lyophilizing the ECM; rehydrating the ECM; and drying the ECM at a temperature of 40° C. to 70° C. to produce said ECM composition, wherein said method does not comprise modification of the ECM with a chemical or protease.