Detection of Non-Nucleic Acid Analytes Using Strand Displacement Exchange Reactions

摘要

The present invention relates to an analyte detection system for detecting analytes different from DNA and RNA. The system comprises a set of oligonucleotides which may hybridize to each other in specific ways and is able to generate a signal based on the specific hybridization events. The system relies on changes in the hybridization equilibrium between the oligonucleotides in the presence of an analyte or analytes, which results in a change in signal.

主权项

1. An analyte detection system for detecting an analyte different from DNA and RNA, the system comprising at least a first oligonucleotide A, a second oligonucleotide B, and a third oligonucleotide S, wherein:
each of oligonucleotides A and B comprise a sequence that is complementary or partly complementary to a sequence on oligonucleotide S, and wherein oligonucleotides A and B compete for hybridization to oligonucleotide S in a dynamic equilibrium, and optionally wherein at least one of oligonucleotides A and B comprises a covalently linked binding moiety capable of interacting with an analyte different from DNA and RNA; and at least one of oligonucleotides A and B, or a covalently linked binding moiety bound to said oligonucleotide, is capable of interacting with an analyte different from DNA and RNA, such that interaction of said analyte with the oligonucleotide or binding moiety results in a shift in the hybridization equilibrium, the shift in equilibrium providing a detectable signal.