| 主权项 |
1. A transgenic soybean resistant to soybean cyst nematode (SCN), the soybean plant transformed with an artificial DNA construct comprising, as operably associated components in the 5′ to 3′ direction of transcription:
a promoter that functions in soybean; a polynucleotide comprising a sequence selected from the group consisting of
(a) SEQ ID NO: 1 or a sequence 95% identical thereto, wherein the polynucleotide encodes a polypeptide having serine hydroxymethyltransferase activity, or a functional fragment thereof;(b) a nucleotide sequence encoding a polypeptide of SEQ ID NO: 2 or a sequence 95% identical thereto having serine hydroxymethyltransferase activity, or a functional fragment thereof;(c) a nucleotide sequence that hybridizes under stringent conditions to a nucleic acid sequence of SEQ ID NO: 1, wherein the polynucleotide encodes a polypeptide having serine hydroxymethyltransferase activity, wherein said stringent conditions comprise incubation at 65° C. in a solution comprising 6×SSC (0.9 M sodium chloride and 0.09 M sodium citrate), or a functional fragment thereof; and(d) a polynucleotide complementary to the polynucleotide sequence of (a), (b), or (c), or a functional fragment thereof; and a transcriptional termination sequence; wherein the transgenic soybean exhibits increased SCN resistance. |
