发明名称 |
Method for Detection of Target Nucleic Acid |
摘要 |
An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information. |
申请公布号 |
US2016032367(A1) |
申请公布日期 |
2016.02.04 |
申请号 |
US201514883043 |
申请日期 |
2015.10.14 |
申请人 |
NGK INSULATORS, LTD. |
发明人 |
Kawase Mitsuo;Hirota Toshikazu;Niwa Kousuke |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
1. A set of primers for use in a method of detection of a target nucleic acid, wherein the set of priers comprises:
a first primer having an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence complementary to a tag sequence, wherein the tag sequence is complementary to a detection probe correlated to the target nucleic acid; and a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence, wherein the set of primers does not include a universal primer. |
地址 |
Aichi-ken JP |