发明名称 |
Mutant Endoglycoceramidases with Enhanced Synthetic Activity |
摘要 |
The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme. |
申请公布号 |
US2016032342(A1) |
申请公布日期 |
2016.02.04 |
申请号 |
US201514754310 |
申请日期 |
2015.06.29 |
申请人 |
Seneb Biosciences, Inc. ;The University of British Columbia |
发明人 |
JOHNSON Karl F.;DEFREES Shawn;WITHERS Stephen;VAUGHAN Mark |
分类号 |
C12P19/26;C12N9/24 |
主分类号 |
C12P19/26 |
代理机构 |
|
代理人 |
|
主权项 |
1. (canceled) |
地址 |
North Wales PA US |