SINGLE CELL BAR-CODING FOR ANTIBODY DISCOVERY

摘要

Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to heavy and light chain antibody sequences originating from a single cell, antibody discovery, disease and immune diagnostics, and low error sequencing.

主权项

1. A method of preparing a library of sequences comprising:
(a) forming a plurality of first vessels each comprising:
(i) a single cell, and(ii) a single solid support; (b) copying onto the single solid support:
(i) a first copy of a first cell polynucleotide from the single cell, and(ii) a second copy of a second cell polynucleotide from the single cell; (c) forming a plurality of second vessels each comprising
(i) a single solid support from the plurality of first vessels, and(ii) a barcoded polynucleotide; and (d) amplifying the first copy, the second copy, and the barcode with
(i) a first set of primers, and(ii) a second set of primers, wherein a primer of the first set is complimentary to a primer of the second set;thereby forming first and second single cell barcoded sequences.