METHODS AND COMPOSITIONS USEFUL IN MANIPULATING THE STABILITY OF RE1 SILENCING TRANSCRIPTION FACTOR

摘要

Disclosed are methods of screening for compounds that promote REST degradation by inhibiting the activity of the CDTSP1 phosphorylase including fluorescent and antibody based screens. Also disclosed are peptides that promote REST stabilization as well as antibodies that recognize REST phosphorylated at serine 861 and serine 864.

主权项

1. A method of selecting a test compound that promotes REST degradation, the method comprising:
adding the test compound to a mixture; adding a polypeptide comprising SEQ ID NO: 1 to the mixture, wherein both serines of SEQ ID NO: 1 are phosphorylated; and adding a protein at least 70% identical to SEQ ID NO: 5 to the mixture; wherein a test compound that causes the maintenance of phosphorylation of the serines of SEQ ID NO: 1 is a compound that promotes REST degradation.