| 摘要 |
Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated. |
| 主权项 |
1. A method of probing multiple targets in a biological sample comprising:
(a) providing a biological sample containing multiple targets; (b) binding multiple probes to multiple targets present in the biological sample and wherein said probe comprises a binder and at least one of an optical signal generator or a fluorescent signal generator wherein the binder is capable of binding specifically to one or more of the targets present in the biological sample; (c) observing a first set of signals from the first set of probes bound in step (b); (d) contacting the sample comprising the bound probe of step (b) with an electron transfer reagent wherein the electron transfer reagent is a borate salt represented by the following structural formula: wherein:each R1, R2, and R3 is, independently, an alkyl, an alkenyl, an akynyl, an aryl or a heteroaryl, wherein said alkyl, alkenyl, alkynyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from the group consisting of (C1-C4)alkyl, (C1-C4)alkoxy, (C1-C4)alkylamino, amino, hydroxyl, cyano, halogen, or nitro:R4 is an alkyl, an alkenyl, or an akynyl, wherein said alkyl, alkenyl, or alkynyl is optionally substituted with one or more substituents selected from the group consisting of (C1-C4)alkyl, (C1-C4)alkoxy, (C1-C4)alkylamino, amino, hydroxyl, cyano, halogen, or nitro: and M+ is selected from the group consisting of organic and inorganic cations; (e) irradiating the biological sample of step (d); (f) generating a second set of signals from the second set of probes bound in step (b); (g) observing the second set of signals. |
