Single cell nucleic acid analysis

摘要

The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

主权项

1. A method for analyzing DNA from a single cell, said method comprising:
(a) performing whole genome amplification of the genome of a single cell to produce an amplified genome, wherein the method entails performing whole genome amplification for fewer than, or about, 10 amplification cycles; (b) preamplifying the amplified genome using a plurality of target-specific primer pairs to produce a preamplification reaction mixture comprising a plurality of amplicons specific for a plurality of target nucleic acids; (c) distributing the plurality of amplicons within a device; and (d) amplifying and detecting the plurality of amplicons in the device.