Method and device for superresolution optical measurement using singular optics

摘要

The invention proposes an optical method of measurement and an optical apparatus for determining the spatial position of at least one luminous nanoemitter of a sample, the method comprising: the projection of a sequence of at least two compact luminous distributions of different topological families onto the sample, the detection of the light reemitted by said at least one luminous nanoemitter of the sample; the generation of at least one optical image for each luminous distribution, on the basis of the light detected; and the algorithmic analysis of the optical images to obtain information regarding the location of said at least one luminous nanoemitter. The invention further relates to an optical method of measurement and an optical apparatus for determining the spatial position of a plurality of point light sources, the method comprising the detection of the light emitted by the plurality of point light sources; and the separation of the light emitted on a plurality of detectors for simultaneous or sequential detections; the proportion of the light emitted by a point light source, channeled to a specific detector, being dependent on the spatial position of said point light source; and the generation of the optical images on the basis of the light detected; and the algorithmic analysis of the optical images to obtain an item of information regarding the location of the plurality of point light sources.

主权项

1. A method of optical measurement for determining the spatial position of at least one luminous object in a sample, the method comprising:
projecting onto the sample a sequence of a first compact luminous distribution and a second compact luminous distribution, wherein the first and second compact luminous distributions are of different topological families; detecting light re-emitted by said at least one luminous object of the sample; generating from the detected light a first image of the at least one luminous object as illuminated by the first compact luminous distribution and a second image of the at least one luminous object as illuminated by the second compact luminous distribution; and algorithmically analyzing images to obtain spatial position information of the at least one luminous object.