Compositions, methods and kits to detect adenovirus nucleic acids

摘要

The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers.

主权项

1. A composition for use in an Adenovirus target nucleic acid amplification assay comprising
(I) at least three amplification oligomers capable of stably hybridizing to Adenovirus target nucleic acid to generate an amplicon during an amplification reaction, wherein the at least three amplification oligomers are
(a) a first amplification oligomer consisting of a target hybridizing sequence as set forth in SEQ ID NO:27,(b) a second amplification oligomer consisting of a target hybridizing sequence as set forth in SEQ ID NO:28, and(c) a third amplification oligomer consisting of target hybridizing sequences as set forth in (i) SEQ ID NO:25 and/or SEQ ID NO:26, (ii) SEQ ID NO:33 and/or SEQ ID NO:34, or (iii) SEQ ID NO:31 and/or SEQ ID NO:26; and (II) at least one labelled detection probe oligonucleotide configured to specifically hybridize to a portion of said amplicon.