| 发明名称 | Protein purification | ||
| 摘要 | A method for purifying a polypeptide by ion exchange chromatography is described which involves changing the conductivity and/or pH of buffers in order to resolve a polypeptide of interest from one or more contaminants. | ||
| 申请公布号 | US9249218(B2) | 申请公布日期 | 2016.02.02 |
| 申请号 | US201113313931 | 申请日期 | 2011.12.07 |
| 申请人 | GENENTECH, INC. | 发明人 | Basey Carol D.;Blank Greg S. |
| 分类号 | A61K39/395;A61K39/00;A61K39/40;C07K16/28;C07K1/18;C07K16/06;C07K16/32;A61K38/00 | 主分类号 | A61K39/395 |
| 代理机构 | Arnold & Porter LLP | 代理人 | Marschang Diane;Dreger Ginger R.;Arnold & Porter LLP |
| 主权项 | 1. A therapeutic composition comprising a mixture of anti-HER2 antibody and one or more acidic variants thereof, wherein the amount of the acidic variant(s) is less than about 25%, and wherein the acidic variant(s) are predominantly deamidated variants wherein one or more asparagine residues of the anti-HER2 antibody have been deamidated, and wherein the anti-HER2 antibody is humMAb4D5-8, and wherein the deamidated variants have Asn30 in CDR1 of either or both VL regions of humMAb4D5-8 converted to aspartate, and a pharmaceutically acceptable carrier. | ||
| 地址 | South San Francisco CA US | ||