Recombinant microorganisms having phosphoketolase activity and diminished phosphofructokinase activity and/or glucose-6 phosphate dehydrogenase activity

摘要

Described are recombinant microorganisms characterized by having phosphoketolase activity, having a diminished or inactivated Embden-Meyerhof-Parnas pathway (EMPP) by inactivation of the gene(s) encoding phosphofructokinase or by reducing phosphofructokinase activity as compared to a non-modified microorganism and having a diminished or inactivated oxidative branch of the pentose phosphate pathway (PPP) by inactivation of the gene(s) encoding glucose-6-phosphate dehydrogenase or by reducing glucose-6-phosphate dehydrogenase activity as compared to a non-modified microorganism. These microorganisms can be used for the production of useful metabolites such as acetone, isobutene or propene.

主权项

1. A genetically modified prokaryotic microorganism comprising:
(a) increased phosphoketolase activity as compared to a non-genetically modified microorganism; and (b) (i) diminished or inactivated phosphofructokinase activity as compared to a non-genetically modified microorganism; or
(ii) not possessing phosphofructokinase activity; and (c) (i) diminished or inactivated glucose-6-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism; or
(ii) not possessing glucose-6-phosphate dehydrogenase activity; and either (d) (i) reduced glyceraldehyde 3-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism; or
(ii) increased fructose-1,6-bisphosphate phosphatase activity when grown on glucose as compared to a non-genetically modified microorganism.