Sensor device with imaging optics

摘要

The invention relates to a method and a sensor device (100) for detecting particles (MP) that are bound to the binding surface (12) of a carrier (11). The sensor device (100) comprises a microscope (50) for imaging bound particles (MP) onto an image sensor (53). In order to increase the spatial resolution of the microscope (50), a displacement unit (60,70,80) is provided that can displace the carrier (11) relative to the image sensor (53). The distance of a bound particle (MP) from the binding surface (12) and/or its lateral displacement in reaction to forces can thus be determine with high accuracy. This allows to discriminate specific bindings of large magnetic particles (MP) that are bound to the binding surface (12) via smaller target particles from nonspecific direct bindings.

主权项

1. A sensor device for detecting label particles at the binding surface of a carrier, the sensor device comprising:
a) a microscope with an image sensor onto which label particles are imaged, at least one label particle being bound to the binding surface of the carrier via a target particle; b) a displacement unit for controllably displacing the image sensor relative to the carrier; and c) an evaluation unit for evaluating images generated by the image sensor at different relative displacements of the carrier and the image sensor to identify the at least one label particle being bound to the binding surface of the carrier via the target particle, indicating presence of the target particle.